NEW Stem Cell Reagents
-
Human Stem Cells
-
CD34 (clone 4H11) -
CD90 (clone 5E10) -
CD96 (clone NK92.39 ) -
CD133 (clone TMP4) -
CD133 (clone EMK08) -
CD325 (N-Cadherin) (clone 8C11) -
Oct 3/4 (clone EM92) -
FITC Hematopoietic Lineage Flow Cocktail
-
-
Mouse Stem Cells
-
CD150 (clone mShad150) -
CD201 (clone eBio1560) -
Delta-Like 1 -
Endomucin (clone V.7C7) -
Recombinant Leukemia Inhibitory Factor (LIF) -
Nanog (clone MLC-51) -
Notch1 -
Notch3 -
Notch4 -
Oct 3/4 (clone EM92) -
Pacific Blue® Hematopoietic Lineage Flow Cocktail
-
Anti-human CD34
 |
Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and Alexa Fluor® 647 Mouse IgG1, K Iso Cntrl (cat. 51-4714) (left) or Alexa Fluor® 647 anti-human CD34 (4H11) (right). Cells in the lymphocyte gate were used for analysis. |
Anti-human CD133
 |
 |
|
|
Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD45 (HI30) (cat. 11-0459) and PE Mouse IgG2b Iso Cntrl (cat. 12-4732) (left) or PE anti-human CD133 / AC133 (EMK08) (right). Cells in the lymphocyte gate were used for analysis. |
293T (lanes 1 and 3) or human CD133-transduced 293T (lanes 2 and 4) cell lysates either non-reduced (lanes 1 and 2) or reduced (lanes 3 and 4) were loaded at 1X105 cells/lane, probed with 2 ug/ml purified EMK08, and revealed with HRP anti-mouse IgG. |
Anti-mouse Nanog
Quantitative Assessment of Embryonic Stem (ES) Cell
Differentiation by Flow Cytometry
 |
Down Regulation of Nanog in Differentiated Cells: Flow cytometric analysis of Nanog in either untreated (left panel) or treated (right panel) F9 cells . Cells were cultured for three days with All-Trans-Retinoic Acid and then stained with either Alexa Fluor® 647 isotype control (Cat. No. 51-4321, open histogram) or Alexa Fluor® 647 Anti-Mouse Nanog (Cat. No. 51-5761, shaded histogram) using the Foxp3 Buffer Set (Cat. No. 00-5523). |
|
ES/iPS Cell Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| β-catenin
|
2567
|
2567
|
| CD9
|
0098
|
0091
|
| CD15
|
0159
|
--
|
| CD24
|
0247
|
0242
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD30
|
--
|
0301
|
| CD49f (Integrin a6)
|
0495
|
0495
|
| CD338 (ABCG2)
|
8888
|
--
|
| c-Kit (CD117)
|
1179
|
1171
|
| c-Myc
|
6785
|
6785
|
| Dll1 (Delta-like 1)
|
--
|
5767
|
| E-Cadherin (CD324)
|
3249
|
3249
|
| Eomes
|
4877
|
4875
|
| EpCAM (TROP-1)
|
9326
|
5791
|
| Jagged-2
|
--
|
3392
|
| Nanog
|
5769
|
5761
|
| Notch1
|
5785
|
5785
|
| Notch3
|
--
|
5763
|
| Notch4
|
--
|
5764
|
| Oct 3/4
|
5841
|
5841
|
| SSEA-1
|
8813
|
8813
|
| SSEA-3
|
8833
|
8833
|
| SSEA-4
|
8843
|
--
|
| STAT3
|
6727
|
6727
|
| TRA-1-60
|
8863
|
--
|
| TRA-1-81
|
8883
|
--
|
|
Ectoderm Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| Nestin
|
9843
|
5843
|
| Notch1
|
5785
|
5785
|
| Neural Markers
|
|
|
| CD24 (HSA)
|
0247
|
0242
|
| CD56 (NCAM)
|
--
|
0569
|
| CD81 (TAPA-1)
|
--
|
0811
|
| CD90 (Thy-1)
|
0909
|
0900
|
| CD166 (ALCAM)
|
--
|
1661
|
| CD325 (N-Cadherin)
|
3259
|
--
|
| Nestin
|
9843
|
5843
|
| Oligodendrocyte Markers
|
|
|
| CD44
|
0441
|
0441
|
| CD140a (PDGFRa)
|
--
|
1401
|
| Neural Crest Markers
|
|
|
| CD49d
|
0499
|
0492
|
| CD57
|
0577
|
--
|
| LPAM-1
|
--
|
5887
|
| Notch1
|
5785
|
5785
|
| Neural Stem Cell Markers
|
|
|
| CD15
|
0159
|
--
|
| CD24 (HSA)
|
0247
|
0242
|
| CD29 (Integrin β1}
|
0299
|
0291
|
| CD49f (Integrin α6)
|
0495
|
0495
|
| CD54 (ICAM-1)
|
0549
|
0541
|
| CD81
|
--
|
0811
|
| CD95 (FAS/APO-1)
|
0959
|
0951
|
| CD133
|
1338
|
1331
|
| CD140a (PDGFRa)
|
--
|
1401
|
| CD146
|
1469
|
--
|
| CD184 (CXCR4)
|
9999
|
9991
|
| CD338 (ABCG2)
|
8888
|
--
|
| Nestin
|
9843
|
5843
|
| Notch1
|
5785
|
5785
|
| SSEA-1
|
8813
|
8813
|
| Skin Precursor Markers
|
|
|
| CD49d
|
0499
|
0492
|
| CD166 (ALCAM)
|
--
|
1661
|
| Foxj1
|
9965
|
9965
|
| LPAM-1
|
--
|
5887
|
| Nestin
|
9843
|
5843
|
| Sca-1 (Ly-6A/E)
|
--
|
5981
|
|
Endoderm Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| β-Catenin
|
2567
|
2567
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD49f (Integrin α6)
|
0495
|
0495
|
| CD90 (Thy-1)
|
0909
|
0900
|
| CD184 (CXCR4)
|
9999
|
9991
|
| c-Met (HGFR)
|
8858
|
8854
|
| GATA-4
|
9980
|
9980
|
| N-Cadherin (CD325)
|
3259
|
--
|
| Sca-1 (Ly-6A/E)
|
--
|
5981
|
|
Mesoderm Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| Endothelial Cell Markers
|
|
|
| CD14
|
0149
|
0141
|
| CD15
|
0159
|
--
|
| CD18 (Integrin β2)
|
0187
|
0181
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD36
|
0369
|
0361
|
| CD45 (LCA)
|
0459
|
0451
|
| CD45R (B220)
|
0452
|
0452
|
| CD49d (Integrin α4)
|
0499
|
0492
|
| CD49e (Integrin α5)
|
0496
|
0493
|
| CD49f (Integrin α6)
|
0495
|
0495
|
| CD54 (ICAM-1)
|
0549
|
0541
|
| CD56 (NCAM)
|
0568
|
--
|
| CD62E (E-selectin)
|
0627
|
--
|
| CD62L (L-selectin)
|
0629
|
0621
|
| CD62P (P-selectin)
|
0628
|
0626
|
| CD93 (C1qRp)
|
0939
|
5892
|
| CD102
|
1029
|
1021
|
| CD105 (Endoglin)
|
1057
|
1051
|
| CD106 (VCAM-1)
|
1069
|
1061
|
| CD109
|
1099
|
--
|
| CD116 (GM-CSFR)
|
1169
|
--
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD120a (TNFR Type I)
|
--
|
1202
|
| CD120b (TNFR Type II)
|
--
|
1203
|
| CD124 (IL-4Rα)
|
1249
|
--
|
| CD144 (VE-Cadherin)
|
1449
|
1441
|
| CD146 (MCAM)
|
1469
|
--
|
| CD147 (Neurothelin)
|
1472
|
1471
|
| CD152 (CTLA-4)
|
1529
|
1522
|
| CD157
|
1579
|
--
|
| CD166 (ALCAM)
|
--
|
1661
|
| CD184 (CXCR4)
|
9999
|
9991
|
| CD201 (EPCR)
|
--
|
2012
|
| CD209 (DC-SIGN)
|
2099
|
2092
|
| CD252 (OX-40L)
|
--
|
5905
|
| CD253 (TRAIL)
|
9927
|
5951
|
| CD262 (TRAIL-R2)
|
9908
|
5883
|
| CD309 (FLK-1, VEGF-R2))
|
--
|
5821
|
| CD325 (N-cadherin)
|
3259
|
--
|
| Nestin
|
9843
|
5843
|
| Sca-1 (Ly-6A/E)
|
--
|
5981
|
| STAT3
|
6727
|
6727
|
| Cardiogenesis Markers
|
|
|
| CD31
|
0319
|
0311
|
| CD34
|
0349
|
0341
|
| CD66a
|
--
|
0661
|
| CD106
|
1069
|
1061
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD144 (VE-Cadherin)
|
1449
|
1441
|
| CD166 (ALCAM)
|
--
|
1661
|
| CD309 (FLK-1, VEGF-R2
|
--
|
5821
|
| CD325 (N-Cadherin)
|
3259
|
--
|
| Gata-4
|
9980
|
9980
|
| Bone Marrow Precursor Markers (positive)
|
|
|
| CD10
|
0106
|
--
|
| CD13
|
0138
|
--
|
| CD14
|
0149
|
0141
|
| CD15
|
0159
|
--
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD49d (Integrin α4)
|
0492
|
0499
|
| CD49e (Integrin α5)
|
0496
|
0493
|
| CD51 (Integrin αV)
|
--
|
0512
|
| CD54 (ICAM-1)
|
0541
|
0549
|
| CD71 (Transferrin Receptor)
|
0719
|
0711
|
| CD73 (Ecto-5'-Nucleotidase)
|
--
|
0731
|
| CD90 (Thy-1)
|
0909
|
0900
|
| CD105 (Endoglin)
|
1057
|
1051
|
| CD106 (VCAM-1)
|
1069
|
1061
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD120a (TNFR Type I)
|
--
|
1202
|
| CD120b (TNFR Type II)
|
--
|
1203
|
| CD166 (ALCAM)
|
--
|
1661
|
| CD309 (Flk-1, VEGF-R2)
|
--
|
5821
|
| Sca-1 (Ly-6A/E)
|
--
|
5981
|
| Bone Marrow Precursor Markers (negative)
|
|
|
| CD11a
|
0119
|
0111
|
| CD11b
|
0118
|
0112
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD45 (LCA)
|
0459
|
0451
|
| Adipose Precursor Markers (positive)
|
|
|
| CD9
|
0098
|
0091
|
| CD10
|
0106
|
--
|
| CD13
|
0138
|
--
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD44
|
0441
|
0441
|
| CD49d (Integrin α4)
|
0499
|
0492
|
| CD49e (Integrin α5)
|
0496
|
0493
|
| CD54 (ICAM-1)
|
0549
|
0541
|
| CD55
|
0559
|
--
|
| CD73 (Ecto-5'-Nucleotidase)
|
--
|
0731
|
| CD90 (Thy-1)
|
0909
|
0900
|
| CD105 (Endoglin)
|
1057
|
1051
|
| CD106 (VCAM-1)
|
1069
|
1061
|
| CD146 (MCAM)
|
1469
|
--
|
| CD166 (ALCAM)
|
--
|
1661
|
| Adipose Precursor Markers (negative)
|
|
|
| CD11b
|
0113
|
0112
|
| CD14
|
0149
|
0141
|
| CD19
|
0198
|
0199
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD45 (LCA)
|
0459
|
0451
|
| CD79a
|
--
|
0791
|
| HLA-DR
|
9956
|
--
|
|
Hematopoietic Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| Lymphoid Markers
|
|
|
| CD10
|
0106
|
--
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD38
|
0388
|
0381
|
| CD90 (Thy-1)
|
0909
|
0901
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD124 (IL-4Ra)
|
1249
|
--
|
| CD127 (IL-7Ra)
|
1278
|
1271
|
| HLA-DR
|
9952
|
--
|
| Megakaryoblast Markers
|
|
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD36
|
0369
|
0361
|
| CD41
|
0418
|
0411
|
| CD61 (Integrin β3)
|
0519
|
0611
|
| HLA-DR
|
9952
|
--
|
| Monoblast Markers
|
|
|
| CD11c
|
0116
|
0114
|
| CD13
|
0138
|
--
|
| CD15
|
0159
|
--
|
| CD33
|
0038
|
--
|
| CD115 (FMS)
|
1159
|
1152
|
| CD116 (GM-CSFR)
|
1169
|
--
|
| Myeloblast Markers
|
|
|
| CD13
|
0138
|
--
|
| CD15
|
0159
|
--
|
| CD33
|
0038
|
--
|
| CD116 (GM-CSFR)
|
1169
|
--
|
| Pro-Erythroblast Markers
|
|
|
| CD71 (Transferrin Receptor)
|
0719
|
0711
|
| CD105 (Endoglin)
|
1057
|
1051
|
| TER-119
|
--
|
5921
|
| HSC Markers (negative)
|
|
|
| CD2
|
0029
|
0021
|
| CD3
|
0039
|
0031
|
| CD4
|
0049
|
0041
|
| CD10
|
0106
|
--
|
| CD13
|
-138
|
--
|
| CD14
|
0149
|
0141
|
| CD15
|
0159
|
--
|
| CD16
|
0168
|
0161
|
| CD19
|
0198
|
0191
|
| CD20
|
0209
|
0201
|
| CD24
|
0247
|
0242
|
| CD33
|
0338
|
--
|
| CD36
|
0369
|
0361
|
| CD38
|
0388
|
0381
|
| CD45 (LCA)
|
0459
|
0451
|
| CD48
|
0489
|
0451
|
| CD56 (NCAM)
|
0568
|
--
|
| CD138 (Sydecan-1)
|
1389
|
--
|
| CD244
|
2449
|
2441
|
| GATA-3
|
9966
|
9966
|
| HSC Markers (positive)
|
|
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD44
|
0441
|
0441
|
| CD84
|
0849
|
--
|
| CD90 (Thy-1)
|
0909
|
0901
|
| CD90.1 (Thy-1.1)
|
--
|
0900
|
| CD93 (C1qRp)
|
0938
|
5892
|
| CD105 (Endoglin)
|
1057
|
1051
|
| CD106 (VCAM-1)
|
1069
|
1061
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD201 (EPCR)
|
--
|
2012
|
| CD150 (SLAM)
|
1509
|
1502
|
| CD184 (CXCR4)
|
9999
|
9991
|
| CD325 (N-Cadherin)
|
3259
|
|
| CD338 (ABCG2)
|
8888
|
--
|
| Flk-1 (VEGF-R2)
|
--
|
5821
|
| Notch1
|
5875
|
5875
|
| Sca-1 (Ly-6A/E)
|
--
|
5981
|
| WASP
|
6987
|
6987
|
| Hemangioblast Markers
|
|
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD144 (VE-Cadherin)
|
1449
|
1441
|
| CD202 (Tie-2)
|
--
|
5987
|
| CD309 (Flk-1, VEGF-R2)
|
--
|
5821
|
| CD324 (E-Cadherin)
|
3249
|
3249
|
|
Cancer Stem Cell Markers |
||
|---|---|---|
| Specificity | Human | Mouse |
| CD7
|
0079
|
--
|
| CD10
|
0106
|
--
|
| CD18 (Integrin β2)
|
0187
|
0181
|
| CD19
|
0199
|
0193
|
| CD20
|
0209
|
0201
|
| CD24 (HSA)
|
0247
|
0242
|
| CD27
|
0279
|
0271
|
| CD29 (Integrin β1)
|
0299
|
0291
|
| CD31 (PECAM-1)
|
0319
|
0311
|
| CD33
|
0338
|
--
|
| CD34 (Mucosialin)
|
0349
|
0341
|
| CD38
|
0389
|
0381
|
| CD44
|
0441
|
0441
|
| CD49b (Integrin α2)
|
0498
|
0491
|
| CD49f (Integrin α6)
|
0495
|
0495
|
| CD74
|
0749
|
--
|
| CD90 (Thy-1)
|
0909
|
0900
|
| CD96 (Tactile)
|
0969
|
--
|
| CD105 (Endoglin)
|
1057
|
1051
|
| CD117 (c-Kit)
|
1179
|
1171
|
| CD123 (IL-3Rα)
|
1239
|
1231
|
| CD133 (Prominin-1)
|
1338
|
1331
|
| CD138 (Syndecan-1)
|
1389
|
--
|
| CD166 (ALCAM)
|
--
|
1661
|
| CD184 (CXCR4)
|
9999
|
9991
|
| CD324 (E-cadherin)
|
3249
|
3249
|
| CD338 (ABCG2)
|
8888
|
--
|
| Dll1
|
--
|
5767
|
| EpCAM (TROP-1)
|
9326
|
5791
|
| Jagged-2
|
--
|
3392
|
| Nestin
|
9843
|
5843
|
| Notch1
|
5785
|
5785
|
| Notch3
|
--
|
5763
|
| Notch4
|
--
|
5764
|
| Podoplanin
|
9381
|
5381
|
| SSEA-1
|
8813
|
8813
|
| SSEA-3
|
8833
|
8833
|
| SSEA-4
|
8843
|
--
|
| TRA-1-60
|
8863
|
--
|
| TRA-1-80
|
8883
|
--
|
|
|
|
|
|
Recombinant Proteins for Stem Cell Culture & Differentiation |
||
|---|---|---|
| Protein | Human | Mouse |
| ES/iPS Cell Culture
|
|
|
| BMP2
|
8507
|
--
|
| EGF
|
8988
|
8984
|
| FGF-acidic
|
8987
|
8981
|
| FGF-basic
|
8986
|
8982
|
| Leukemia Inhibitory Factor (LIF)
|
--
|
8521
|
| PDGF-AA
|
--
|
8989
|
| PDGF-BB
|
8501
|
--
|
| TGFβ
|
8348
|
--
|
| TGFβ2
|
8368
|
--
|
| Ectoderm Cell Culture
|
|
|
| BMP2
|
8507
|
--
|
| EGF
|
8988
|
|
| GDNF
|
8506
|
--
|
| IGF-1
|
--
|
8508
|
| IGF-2
|
8505
|
--
|
| NGF-b
|
--
|
8504
|
| PDGF-AA
|
--
|
8989
|
| PDGF-BB
|
8501
|
--
|
| TGFβ
|
8348
|
--
|
| TGFβ2
|
8368
|
--
|
| Endoderm Cell Culture
|
|
|
| BMP2
|
8507
|
--
|
| EGF
|
8988
|
8984
|
| HGF
|
8439
|
8431
|
| IGF-1
|
--
|
8508
|
| IGF-2
|
8505
|
--
|
| IL-6
|
7066
|
7064
|
| PDGF-AA
|
--
|
8989
|
| PDGF-BB
|
8501
|
--
|
| SCF
|
8349
|
8341
|
| TGFβ
|
8348
|
--
|
| TGFβ2
|
8368
|
--
|
| VEGF
|
8359
|
8351
|
| Mesoderm Cell Culture
|
|
|
| BMP2
|
8507
|
--
|
| CTGF
|
8503
|
--
|
| G-CSF
|
8979
|
8971
|
| GM-CSF
|
8339
|
8331
|
| HGF
|
8439
|
8431
|
| IGF-1
|
--
|
8508
|
| IGF-2
|
8505
|
--
|
| IL-6
|
8069
|
8061
|
| Leptin
|
8509
|
8510
|
| M-CSF
|
8985
|
8983
|
| PDGF-AA
|
--
|
8989
|
| PDGF-BB
|
8501
|
--
|
| SCF
|
8349
|
8341
|
| TGFβ
|
8348
|
--
|
| TGFβ2
|
8368
|
--
|
| VEGF
|
8359
|
8351
|
| HSC Cell Culture
|
|
|
| BMP2
|
8507
|
--
|
| Flt-3L
|
8513
|
--
|
| G-CSF
|
8979
|
8971
|
| GM-CSF
|
8339
|
8331
|
| HGF
|
8439
|
8431
|
| IGF-1
|
--
|
8508
|
| IGF-2
|
8505
|
--
|
| IL-3
|
8039
|
8031
|
| IL-6
|
8069
|
8061
|
| IL-7
|
8079
|
8071
|
| M-CSF
|
8985
|
8983
|
| SCF
|
8349
|
8341
|
| TGFβ
|
8348
|
--
|
| TGFβ2
|
8648
|
--
|
| TNFα
|
8329
|
8321
|
| TSLP
|
8497
|
8498
|
| VEGF
|
8359
|
8351
|
